@article {3171, title = {Imaging bacterial 3D motion using digital in-line holographic microscopy and correlation-based de-noising algorithm.}, journal = {Opt Express}, volume = {22}, number = {26}, year = {2014}, pages = {32119-32137}, abstract = {Better understanding of bacteria environment interactions in the context of biofilm formation requires accurate 3-dimentional measurements of bacteria motility. Digital Holographic Microscopy (DHM) has demonstrated its capability in resolving 3D distribution and mobility of particulates in a dense suspension. Due to their low scattering efficiency, bacteria are substantially difficult to be imaged by DHM. In this paper, we introduce a novel correlation-based de-noising algorithm to remove the background noise and enhance the quality of the hologram. Implemented in conjunction with DHM, we demonstrate that the method allows DHM to resolve 3-D\ E. coli\ bacteria locations of a dense suspension (\>107\ cells/ml) with submicron resolutions (\<0.5\ {\textmu}m) over substantial depth and to obtain thousands of 3D cell trajectories.}, url = {https://www.osapublishing.org/oe/abstract.cfm?uri=oe-22-26-32119}, author = {Molaei, M. and Sheng, J.} }